RT PCR Test Procedure
Below mentioned are the procedures that we follow as standard guidelines that are helpful in the diagnosis of COVID19.
Step 1: The COVID-19 infection, by and large, accumulates in the throat or inside the nose of an individual. The RT-PCR Test conclusion measure begins with test assortment by swab from the referenced body parts.
Step 2: The cells and nucleus are examined by treating them with various substance arrangements. At last, the example just contains DNA and RNA. It is a blend of the lingering genetic material of the individual and the RNA of the infection.
Step 3: With reverse transcription, RNA is converted to DNA. Specific enzymes are used in this procedure. Short fragments of DNA are additionally added to make a mixture.
Step 4: If the virus is present in the sample, the short fragments of the DNA accord to the target divisions of the viral DNA.
Step 5: With the help of RT-PCR machine, the mixture is then heated and cooled through the cycle of temperature to ignite some chemical reactions.
Step 6: In the RT-PCR machine, the number of cycles of temperature is carried out to copy the target sections of the viral DNA. In each cycle, the number of copies is doubled. At the end of the process, 35 billion copies of viral DNA sections from each strand of the virus are generated.